Methodology. (Last updated: 1/25/25)
| Rank | Player | Years | Country |
| 1 | Serena Williams | 1995-2022 | USA |
| 2 | Steffi Graf | 1982-1999 | Germany |
| 3 | Martina Navratilova | 1974-2006 | USA |
| 4 | Chris Evert | 1972-1989 | USA |
| 5 | Margaret Court | 1960-1977 | Australia |
| 6 | Monica Seles | 1989-2003 | Yugoslavia/USA |
| 7 | Helen Wills Moody | 1922-1938 | USA |
| 8 | Billie Jean King | 1968-1990 | USA |
| 9 | Martina Hingis | 1994-2017 | Switzerland |
| 10 | Justine Henin | 1999-2011 | Belgium |
| 11 | Venus Williams | 1994-active | USA |
| 12 | Maria Sharapova | 2001-2020 | Russia |
| 13 | Maureen Connolly | 1949-1954 | USA |
| 14 | Maria Bueno | 1957-1977 | Brazil |
| 15 | Evonne Goolagong Cawley | 1967-1983 | Australia |
| 16 | Iga Świątek | 2016-active | Poland |
| 17 | Lindsay Davenport | 1993-2010 | USA |
| 18 | Arantxa Sánchez Vicario | 1985-2002 | Spain |
| 19 | Kim Clijsters | 1997-2022 | Belgium |
| 20 | Suzanne Lenglen | 1914-1926 | France |
| 21 | Pauline Betz | 1939-1946 | USA |
| 22 | Doris Hart | 1940-1955 | USA |
| 23 | Louise Brough | 1942-1959 | USA |
| 24 | Hana Mandlíková | 1978-1990 | Czechoslovakia/Australia |
| 25 | Simona Halep | 2006-active | Romania |
| 26 | Aryna Sabalenka | 2015-active | Belarus |
| 27 | Gabriela Sabatini | 1982-1996 | Argentina |
| 28 | Jennifer Capriati | 1990-2004 | USA |
| 29 | Ashleigh Barty | 2010-2022 | Australia |
| 30 | Angelique Kerber | 2003-active | Germany |
| 31 | Naomi Osaka | 2013-active | Japan |
| 32 | Margaret Osbourne duPont | 1938-1962 | USA |
| 33 | Victoria Azarenka | 2003-active | Belarus |
| 34 | Petra Kvitová | 2006-active | Czech Republic |
| 35 | Conchita Martinez | 1998-2006 | Spain |
| 36 | Amélie Mauresmo | 1993-2009 | France |
| 37 | Caroline Wozniacki | 2005-active | Denmark |
| 38 | Mary Pierce | 1989-2006 | France |
| 39 | Ann Jones | 1955-1977 | England |
| 40 | Virginia Wade | 1968-1986 | England |
| 41 | Svetlana Kuznetsova | 2000-2021 | Russia |
| 42 | Alice Marble | 1931-1940 | USA |
| 43 | Althea Gibson | 1950-1958 | USA |
| 44 | Shirley Fry | 1941-1957 | USA |
| 45 | Jana Novotná | 1987-1999 | Czech Republic |
| 46 | Garbiñe Muguruza | 2012-2024 | Spain |
| 47 | Darlene Hard | 1953-1970 | USA |
| 48 | Helen Jacobs | 1925-1941 | USA |
| 49 | Hilde Krahwinkel Sperling | 1929-1939 | Germany |
| 50 | Jelena Janković | 2000-2022 | Serbia |
| 51 | Tracy Austin | 1978-1994 | USA |
| 52 | Li Na | 1999-2014 | China |
| 53 | Ana Ivanovic | 2003-2016 | Serbia |
| 54 | Dinara Safina | 2000-2011 | Russia |
| 55 | Madison Keys | 2009-active | USA |
| 56 | Barbora Krejčíková | 2010-active | Czech Republic |
| 57 | Lottie Dod | 1887-1893 | England |
| 58 | Dorothea Douglass Lambert Chambers | 1903-1925 | England |
| 59 | Molla Mallory | 1909-1929 | Norway/USA |
| 60 | Nancy Richey | 1958-1978 | USA |
| 61 | Lesley Bowrey | 1959-1978 | Australia |
| 62 | Angela Mortimer | 1951-1962 | England |
| 63 | Coco Gauf | 2018-active | USA |
| 64 | Karolína Plíšková | 2009-active | Czech Republic |
| 65 | Elena Dementieva | 1998-2010 | Russia |
| 66 | Vera Zvonareva | 2000-active | Russia |
| 67 | Anastasia Myskina | 1998-2007 | Russia |
| 68 | Iva Majoli | 1991-2004 | Croatia |
| 69 | Elena Rybakina | 2014-active | Kazakhstan |
| 70 | Samantha Stosur | 1999-2022 | Australia |
| 71 | Helena Suková | 1983-1998 | Czech Republic |
| 72 | Mary Joe Fernandez | 1986-2000 | USA |
| 73 | Wendy Turnbull | 1975-1989 | Australia |
| 74 | Jelena Ostapenko | 2012-active | Latvia |
| 75 | Sloane Stephens | 2009-acitve | USA |
| 76 | Marion Bartoli | 2000-2013 | France |
| 77 | Flavia Pennetta | 2000-2015 | Italy |
| 78 | Bianca Andreescu | 2017-active | Canada |
| 79 | Markéta Vondroušová | 2017-active | Czech Republic |
| 80 | Simonne Mathieu | 1925-1946 | France |
| 81 | Dorothy Round | 1928-1939 | England |
| 82 | Christine Truman | 1957-1974 | England |
| 83 | Pam Shriver | 1979-1997 | USA |
| 84 | Ons Jabeur | 2010-active | Tunisia |
| 85 | Sofia Kenin | 2017-active | USA |
| 86 | Francesca Schiavone | 1998-2018 | Italy |
| 87 | Andrea Jaeger | 1980-1985 | USA |
| 88 | Françoise Dürr | 1960-1979 | France |
| 89 | Sue Barker | 1972-1984 | England |
| 90 | Mima Jaušovec | 1975-1988 | Yugoslavia |
| 91 | Rosemary Casals | 1964-1985 | USA |
| 92 | Kerry Melville | 1963-1979 | Australia |
| 93 | Margaret Scriven | 1930-1947 | England |
| 94 | Zina Garrison | 1980-1996 | USA |
| 95 | Virginia Ruzici | 1975-1987 | Romania |
| 96 | Olga Morozova | 1965-1979 | Soviet Union |
| 97 | Jasmine Paolini | 2011-active | Italy |
| 98 | Renáta Tomanová | 1973-1986 | Czechoslovakia |
| 99 | Yola Ramirez | 1955-1963 | Mexico |
| 100 | Claudia Kohde-Kilsch | 1980-1994 | Germany |